A Study Of Non-Coding RNAs (ncRNAs) Of Salmonella Typhi Derived From Salmonella Pathogenicity Islands Under Different Stress Conditions
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Date
2017-06
Authors
Saw, Priscilla Cheah Phei
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Non-coding RNAs can be found in all bacteria including Salmonella
Typhi. The Salmonella Typhi is infamous for infecting humans and causing
typhoid fever, a systemic disease that may be mortal to humans. It is able to
survive in various environments including in macrophags. Salmonella Typhi is
similar to other bacteria by consisting of non-coding RNAs that play crucial
roles in its biological pathways in response to environmental. One of the aims in
this study is to investigate non-coding RNAs of Salmonella Typhi in
differentiated THP-1 cells to find novel non-coding RNA expressions during
invasion of macrophages via transcriptome sequencing. However, the
investigation was later suspended due to the challenge of extracting S. Typhi
RNA away from the large RNA pool of the differentiated THP-1 cells,
hampering the transcriptome analysis of S. Typhi’s RNAs. Therefore alternative
approached was studied where the expressions of 10 specific ncRNAs under
different stress were investigated using Northern blot technique, as well as
IntaRNA software to predict their target mRNAs, and RNAfold software to
determine their secondary structures. The various stresses were conditions to
compare with cell growth under normal conditions. Based on the results, StyR-9
and StyR-161 were found to be involved in S. Typhi’s virulence mechanism, by
interacting with rfbI and fur mRNA, respectively. StyR-381 was found to
interact with rbsA mRNA that is involved in ABC transporter formation, which
may be indirectly involved with the virulence mechanism.
Description
Keywords
Non-coding RNAs can be found in all bacteria , including Salmonella Typhi