A Study Of Non-Coding RNAs (ncRNAs) Of Salmonella Typhi Derived From Salmonella Pathogenicity Islands Under Different Stress Conditions

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dc.contributor.authorSaw, Priscilla Cheah Phei
dc.date.accessioned2019-08-09T07:29:09Z
dc.date.available2019-08-09T07:29:09Z
dc.date.issued2017-06
dc.description.abstractNon-coding RNAs can be found in all bacteria including Salmonella Typhi. The Salmonella Typhi is infamous for infecting humans and causing typhoid fever, a systemic disease that may be mortal to humans. It is able to survive in various environments including in macrophags. Salmonella Typhi is similar to other bacteria by consisting of non-coding RNAs that play crucial roles in its biological pathways in response to environmental. One of the aims in this study is to investigate non-coding RNAs of Salmonella Typhi in differentiated THP-1 cells to find novel non-coding RNA expressions during invasion of macrophages via transcriptome sequencing. However, the investigation was later suspended due to the challenge of extracting S. Typhi RNA away from the large RNA pool of the differentiated THP-1 cells, hampering the transcriptome analysis of S. Typhi’s RNAs. Therefore alternative approached was studied where the expressions of 10 specific ncRNAs under different stress were investigated using Northern blot technique, as well as IntaRNA software to predict their target mRNAs, and RNAfold software to determine their secondary structures. The various stresses were conditions to compare with cell growth under normal conditions. Based on the results, StyR-9 and StyR-161 were found to be involved in S. Typhi’s virulence mechanism, by interacting with rfbI and fur mRNA, respectively. StyR-381 was found to interact with rbsA mRNA that is involved in ABC transporter formation, which may be indirectly involved with the virulence mechanism.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8612
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectNon-coding RNAs can be found in all bacteriaen_US
dc.subjectincluding Salmonella Typhien_US
dc.titleA Study Of Non-Coding RNAs (ncRNAs) Of Salmonella Typhi Derived From Salmonella Pathogenicity Islands Under Different Stress Conditionsen_US
dc.typeThesisen_US
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