Molecular Characterization And Production Of A Specific Recombinant Protein Of Shigella Flexneri: Towards Development Of A Rapid Immunochromatography Diagnostic Test For Dysentery
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Date
2006-02
Authors
Banga Singh, Kirnpal Kaur
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Abstract
Bacillary dysentery is caused mainly by infection with Shigella spp. which is also known
as shigellosis. It remains a common and serious health problem throughout the world
and has been estimated to infect about 165 million people worldwide annually. It is an
acute invasive enteric infection often characterized by abdominal pain, fever and
bloody diarrhea. S. flexneri is the most frequently isolated species causing endemic
shigellosis in developing countries. The current method of diagnosis still depends on
the traditional culture method, which is laborious, relatively insensitive and takes 3 to 5
days to produce result. To date, no rapid diagnostic methods are available
commercially. Hence there is a need to develop a rapid, simple, sensitive and specific
diagnostic test, which can diagnose S. flexneri at early stages of infection. A rapid and
reliable diagnostic assay would significantly improve effective treatment, management
and control of this highly infectious bacterium. There is a need for the development of
the next generation immunoassay platform which provides a more rapid, sensitive and
portable point-of-care assay.
In the effort to improve the diagnosis of shigellosis, a specific and antigenic protein of
35 kDa in size has been previously identified which has a diagnostic potential for
detection of Shigella infections. The aim of this study was to develop important
fundamental parameters to generate sufficient amounts of purified recombinant 35 kDa
protein and to exploit its application in developing a rapid dipstick test, as an alternative
diagnostic test for the detection of anti-So flexneri IgA antibody.
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Keywords
Shigella Flexneri , Dysentery