Molecular Characterization And Production Of A Specific Recombinant Protein Of Shigella Flexneri: Towards Development Of A Rapid Immunochromatography Diagnostic Test For Dysentery
| dc.contributor.author | Banga Singh, Kirnpal Kaur | |
| dc.date.accessioned | 2016-10-24T04:33:12Z | |
| dc.date.available | 2016-10-24T04:33:12Z | |
| dc.date.issued | 2006-02 | |
| dc.description.abstract | Bacillary dysentery is caused mainly by infection with Shigella spp. which is also known as shigellosis. It remains a common and serious health problem throughout the world and has been estimated to infect about 165 million people worldwide annually. It is an acute invasive enteric infection often characterized by abdominal pain, fever and bloody diarrhea. S. flexneri is the most frequently isolated species causing endemic shigellosis in developing countries. The current method of diagnosis still depends on the traditional culture method, which is laborious, relatively insensitive and takes 3 to 5 days to produce result. To date, no rapid diagnostic methods are available commercially. Hence there is a need to develop a rapid, simple, sensitive and specific diagnostic test, which can diagnose S. flexneri at early stages of infection. A rapid and reliable diagnostic assay would significantly improve effective treatment, management and control of this highly infectious bacterium. There is a need for the development of the next generation immunoassay platform which provides a more rapid, sensitive and portable point-of-care assay. In the effort to improve the diagnosis of shigellosis, a specific and antigenic protein of 35 kDa in size has been previously identified which has a diagnostic potential for detection of Shigella infections. The aim of this study was to develop important fundamental parameters to generate sufficient amounts of purified recombinant 35 kDa protein and to exploit its application in developing a rapid dipstick test, as an alternative diagnostic test for the detection of anti-So flexneri IgA antibody. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/2841 | |
| dc.subject | Shigella Flexneri | en_US |
| dc.subject | Dysentery | en_US |
| dc.title | Molecular Characterization And Production Of A Specific Recombinant Protein Of Shigella Flexneri: Towards Development Of A Rapid Immunochromatography Diagnostic Test For Dysentery | en_US |
| dc.type | Thesis | en_US |
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