Synthesis And Characterisation Of PEG-PLGA Nanoparticles For Geraniin Isolated From Phyllanthus watsonii (Dukung Anak) Extract
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Date
2018-08
Authors
Jairaman, Sreegayathri
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
A growing number of drug delivery nowadays utilises nanomedicine
technology. The efficiency of drug delivery to the target site in the body depends on
the physico-chemical factors of the drug carrier. Phyllanthus watsonii, which is
locally known as dukung anak, is found in tropical forests in Malaysia. Extracts from
the plants have anti-hypertensive, anti-viral and anti-diabetes properties. Geraniin, as
the major active compound from P.watsonii extract was utilized in the study which is
given by Dr. Indu S Jaganath from Malaysia Agricultural Research and Development
Institute (MARDI). However, the bioavailability of the phytochemicals during drug
delivery are often hampered from the degradation of bare phytochemicals by the pH
and enzymatic actions in the gastrointestinal tract before transcending into the
circulatory system. Thus, this research aims to 1. Synthesize
Poly(ethyleneglycol)-poly(lactic-co-glycolicacid) (PLGA-PEG) based nanoparticles
encapsulated with geraniin which are less than 200 nm in diameter using double
emulsion method, 2. Characterize the physicochemical properties and morphology of
PEG-PLGA nanoparticles, 3. Measure the amount of geraniin encapsulated in the
PEG-PLGA nanoparticles, 4. Identify the in vitro stability and cytotoxicity of
PEG-PLGA nanoparticles and PEG-PLGA nanoparticles encapsulated with geraniin
on normal colon human epithelial cell line, 5. Determine the effect of cellular
organization on cellular uptake. Polymeric nanoparticles (PEG-PLGA) were
synthesized using double emulsion method. In vitro drug release test was employed
to determine the release of active compound, geraniin from the nanoparticles in a
controlled environment. CRL1790 (also known as, CCD 841 CoN) which is
normal colon human epithelial cell line was used as the cell model.
Description
Keywords
Characterisation of PEG-PLGA nanoparticles , geraniin isolated from Phyllanthus watsonii