Synthesis And Characterisation Of PEG-PLGA Nanoparticles For Geraniin Isolated From Phyllanthus watsonii (Dukung Anak) Extract

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dc.contributor.authorJairaman, Sreegayathri
dc.date.accessioned2019-07-17T01:23:14Z
dc.date.available2019-07-17T01:23:14Z
dc.date.issued2018-08
dc.description.abstractA growing number of drug delivery nowadays utilises nanomedicine technology. The efficiency of drug delivery to the target site in the body depends on the physico-chemical factors of the drug carrier. Phyllanthus watsonii, which is locally known as dukung anak, is found in tropical forests in Malaysia. Extracts from the plants have anti-hypertensive, anti-viral and anti-diabetes properties. Geraniin, as the major active compound from P.watsonii extract was utilized in the study which is given by Dr. Indu S Jaganath from Malaysia Agricultural Research and Development Institute (MARDI). However, the bioavailability of the phytochemicals during drug delivery are often hampered from the degradation of bare phytochemicals by the pH and enzymatic actions in the gastrointestinal tract before transcending into the circulatory system. Thus, this research aims to 1. Synthesize Poly(ethyleneglycol)-poly(lactic-co-glycolicacid) (PLGA-PEG) based nanoparticles encapsulated with geraniin which are less than 200 nm in diameter using double emulsion method, 2. Characterize the physicochemical properties and morphology of PEG-PLGA nanoparticles, 3. Measure the amount of geraniin encapsulated in the PEG-PLGA nanoparticles, 4. Identify the in vitro stability and cytotoxicity of PEG-PLGA nanoparticles and PEG-PLGA nanoparticles encapsulated with geraniin on normal colon human epithelial cell line, 5. Determine the effect of cellular organization on cellular uptake. Polymeric nanoparticles (PEG-PLGA) were synthesized using double emulsion method. In vitro drug release test was employed to determine the release of active compound, geraniin from the nanoparticles in a controlled environment. CRL1790 (also known as, CCD 841 CoN) which is normal colon human epithelial cell line was used as the cell model.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8509
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectCharacterisation of PEG-PLGA nanoparticlesen_US
dc.subjectgeraniin isolated from Phyllanthus watsoniien_US
dc.titleSynthesis And Characterisation Of PEG-PLGA Nanoparticles For Geraniin Isolated From Phyllanthus watsonii (Dukung Anak) Extracten_US
dc.typeThesisen_US
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