Antioxidant, anti-angiogenic and antiinflammatory activities of standardized Gynura Segetum leaf extracts
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Date
2014-09
Authors
Seow, Lay Jing
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Abstract
Gynura segetum (daun dewa in Malay) is a herb, reputed of value among others for
treatment of inflammation and cancer. In the present study, the anti-inflammatory
activity of the standardized leaf extracts of Gynura segetum were investigated as well
as its possible relation to antioxidant, anti-angiogenic and cytotoxic activities. The
ethyl acetate fraction showed the strongest antioxidant activity based on DPPH
scavenging activity and beta carotene bleaching capacity assays and directly related
to its total phenolic and flavonoid contents. The methanol extract was the most
active in inhibiting the blood vessels growth in chick embryo chorioallantoic
membrane (CAM) assay and rat aortic ring assay. It shows it has anti-angiogenic
activity. It is non-cytotoxic and suppression of vascular endothelial growth factor
(VEGF) activity is one of the anti-angiogenic mechanisms of action. The methanol
extract possesses anti-inflammatory activity as it inhibited SDS-induced irritation in
hen’s egg chorioallantoic membrane (HET-CAM) test and cotton pellet-induced
granuloma formation in rat. Its anti-inflammatory activity is mediated through the
inhibition of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and
interleukin-1 (IL-1) and cycloxygenase-2 (COX-2) enzyme activities. Taken
together, the present study suggests that Gynura segetum’s leaf is a natural source of
antioxidants and possesses anti-angiogenic activity which may explain its chronic
anti-inflammatory activity and anti-cancer potential.
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Keywords
Gynura Segetum Leaf