Biological Activities Of Acanthophora Spicifera (VAHL) Borgesen From Pulau Gedung, Penang
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Date
2011-06
Authors
Zakaria, Nurul Aili
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
A total of eight extract of Acanthophora spicifera extracted using two
different methods using solvents of different polarity, were used throughout this
study. These extracts were assayed to determine the antimicrobial activities,
antioxidant activities, in-vitro cytotoxicity and total phenolic contents. Most of the
extracts exhibited antibacterial activity against the tested bacteria. Meanwhile, no
antifungal activity was observed on tested fungi. Among the extract, crude methanol
extract demonstrated promising antibacterial activity against Gram-negative
Pseudomonas aeruginosa ATCC 27853 with inhibition zone of 11.0 mm, MIC
values of 250 μg mL-1 and MBC value of 500 μg mL-1. A. spicifera also exhibited
antioxidant properties. Chloroform extract was found to exhibit the highest DPPH
free radical scavenging activity with LC50 of 789 μg mL-1, while standard quercetin
used is 3.361 μg mL-1. Meanwhile, for total phenolic contents, ethyl acetate extract
gave the highest phenol contents (40.58 ± 1.16 μg GAE mg-1 dry extract). All
extracts also showed good positive linear correlation between the antioxidant
activities and total phenolic content, proving the role of phenolics as the main
compounds for antioxidants. Crude methanol extract also exhibited significant
toxicity effects on brine shrimp, Artemia salina with LC50 value obtained for acute
and chronic was 635.47 μg mL-1 and 275.72 μg mL-1, respectively. The crude
methanol extract was later fractionated using silica gel column chromatography
using a mixture of hexane and ethyl acetate as the mobile phase, yielding a total of
15 fractions (af1-af15). Only three fractions (af14, af15 and af7) were found active
against P. aeruginosa ATCC 27853 with the MICs ranging from 125-250 μg ml-1.
Description
Keywords
Biological activities of Acanthophora spicifera , Borgesen from Pulau Gedung, Penang