Biological Activities Of Acanthophora Spicifera (VAHL) Borgesen From Pulau Gedung, Penang

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dc.contributor.authorZakaria, Nurul Aili
dc.date.accessioned2018-12-19T06:50:49Z
dc.date.available2018-12-19T06:50:49Z
dc.date.issued2011-06
dc.description.abstractA total of eight extract of Acanthophora spicifera extracted using two different methods using solvents of different polarity, were used throughout this study. These extracts were assayed to determine the antimicrobial activities, antioxidant activities, in-vitro cytotoxicity and total phenolic contents. Most of the extracts exhibited antibacterial activity against the tested bacteria. Meanwhile, no antifungal activity was observed on tested fungi. Among the extract, crude methanol extract demonstrated promising antibacterial activity against Gram-negative Pseudomonas aeruginosa ATCC 27853 with inhibition zone of 11.0 mm, MIC values of 250 μg mL-1 and MBC value of 500 μg mL-1. A. spicifera also exhibited antioxidant properties. Chloroform extract was found to exhibit the highest DPPH free radical scavenging activity with LC50 of 789 μg mL-1, while standard quercetin used is 3.361 μg mL-1. Meanwhile, for total phenolic contents, ethyl acetate extract gave the highest phenol contents (40.58 ± 1.16 μg GAE mg-1 dry extract). All extracts also showed good positive linear correlation between the antioxidant activities and total phenolic content, proving the role of phenolics as the main compounds for antioxidants. Crude methanol extract also exhibited significant toxicity effects on brine shrimp, Artemia salina with LC50 value obtained for acute and chronic was 635.47 μg mL-1 and 275.72 μg mL-1, respectively. The crude methanol extract was later fractionated using silica gel column chromatography using a mixture of hexane and ethyl acetate as the mobile phase, yielding a total of 15 fractions (af1-af15). Only three fractions (af14, af15 and af7) were found active against P. aeruginosa ATCC 27853 with the MICs ranging from 125-250 μg ml-1.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7341
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectBiological activities of Acanthophora spiciferaen_US
dc.subjectBorgesen from Pulau Gedung, Penangen_US
dc.titleBiological Activities Of Acanthophora Spicifera (VAHL) Borgesen From Pulau Gedung, Penangen_US
dc.typeThesisen_US
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