In Silico Reconstruction Of Biofilm Formation Pathways And Identification Of Putative Biofilmrelated Small Rnas Of Salmonella Enterica Serovar Typhi
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Date
2011-02
Authors
Ng, Fui Ling
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Salmonella enterica serovar Typhi causes typhoid fever and forms biofilm
in the human gallbladder. Biofilm is a complex aggregation of microorganisms
adhering and growing on the surface of a structure. The Pathway Studio software
was used to map out three major components of S. Typhi’s biofilm formation
pathways (exopolysaccharide, lipopolysaccharide, oligosaccharide and O-antigen
chain). Comparative analysis of biofilm formation was performed among S.
Typhi CT18, Salmonella enterica serovar Typhimurium LT2, and Pseudomonas
aeruginosa PAO1 to determine the conserved regulatory pathways. It was
established that quorum sensing, flagella synthesis, multidrug efflux system, curli
biosynthesis, and the two-component signaling system are conserved among
these three microorganisms. The reconstructed pathways showed that CsgD
regulates the production of two major exopolysaccharides (cellulose and curli),
and inhibits the formation of flagellum through cGpGp (also known as c-di-
GMP). The protein SdiA was found to regulate fimbriae and flagella, adherence
on epithelial cells, multidrug tolerance, and quorum sensing. A total of 21
biofilm- related putative small RNAs were identified and isolation was done using
S. Typhimurium ATCC 14028.
Description
Keywords
Biofilm Formation Pathways , Salmonella Enterica Serovar Typhi