In Silico Reconstruction Of Biofilm Formation Pathways And Identification Of Putative Biofilmrelated Small Rnas Of Salmonella Enterica Serovar Typhi

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dc.contributor.authorNg, Fui Ling
dc.date.accessioned2019-10-08T03:33:01Z
dc.date.available2019-10-08T03:33:01Z
dc.date.issued2011-02
dc.description.abstractSalmonella enterica serovar Typhi causes typhoid fever and forms biofilm in the human gallbladder. Biofilm is a complex aggregation of microorganisms adhering and growing on the surface of a structure. The Pathway Studio software was used to map out three major components of S. Typhi’s biofilm formation pathways (exopolysaccharide, lipopolysaccharide, oligosaccharide and O-antigen chain). Comparative analysis of biofilm formation was performed among S. Typhi CT18, Salmonella enterica serovar Typhimurium LT2, and Pseudomonas aeruginosa PAO1 to determine the conserved regulatory pathways. It was established that quorum sensing, flagella synthesis, multidrug efflux system, curli biosynthesis, and the two-component signaling system are conserved among these three microorganisms. The reconstructed pathways showed that CsgD regulates the production of two major exopolysaccharides (cellulose and curli), and inhibits the formation of flagellum through cGpGp (also known as c-di- GMP). The protein SdiA was found to regulate fimbriae and flagella, adherence on epithelial cells, multidrug tolerance, and quorum sensing. A total of 21 biofilm- related putative small RNAs were identified and isolation was done using S. Typhimurium ATCC 14028.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8972
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectBiofilm Formation Pathwaysen_US
dc.subjectSalmonella Enterica Serovar Typhien_US
dc.titleIn Silico Reconstruction Of Biofilm Formation Pathways And Identification Of Putative Biofilmrelated Small Rnas Of Salmonella Enterica Serovar Typhien_US
dc.typeThesisen_US
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