Studies On Standardization Of Ficus Deltoidea Jack And Orthosiphon Stamineus Benth. Leaf Extracts For Selected Anti-Hypertensive And Anti-Angiogenic Effects
Loading...
Date
2012-06
Authors
Darestani, Armaghan Shafaei
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Ficus deltoidea (FD) and Orthosiphon stamineus (OS) were used traditionally
for treatment of a wide range of diseases related to diabetes, hypertension,
inflammation and angiogenesis related diseases in Malaysia. Therefore, this study
was aimed to generate data and evidence about standardization of two medicinal
plants namely Ficus deltoidea (FD) and Orthosiphon stamineus (OS) for antihypertensive
and anti-angiogenesic effects, since there is lack of sufficient scientific
data in this regard.
In this study, gravimetric analysis and microbial limit test (MLT) were applied
on the dried powder of the leaves to examine the quality and safety of the raw
material. Both FD and OS leaves were found to be qualified in terms of
physicochemical properties as well as microbial contamination. Preliminary
phytochemical screening on methanol and water extracts of FD and OS leaves
showed the presence of phenolics, amino acids, flavonoids, terpenoids and saponins
in FD and OS extracts. OS extracts also showed the presence of sterols.
The chemical profile involving qualitative analysis by spectroscopic (UV, IR,
NIR) and chromatographic techniques (HPTLC and GC-MS) and quantitative
analysis by chromatographic techniques (HPTLC and HPLC) were carried out for
standardization. Vitexin, isovitexin, L-citrulline, L-arginine and ursolic acid were
used as markers for the analytical study of FD while, rosmarininc acid, 3-hydroxy-
5,6,7,4-methoxyflavone, sinensetin, eupatorin, oleanolic acid and ursolic acid were
selected as markers for OS based on their medicinal values and their quantifiable
levels.
Description
Keywords
Standardization of ficus deltoidea jack and orthosiphon stamineus benth , for selected anti-hypertensive and anti-angiogenic effects