Electrokinetic Supercharging In Capillary Electrophoresis For The On-Line Preconcentration Of Secbumeton In Water Samples
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Date
2017
Authors
Soeung, Rasmey
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Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
In this study, an on-line preconcentration method termed electrokinetic supercharging capillary zone electrophoresis (EKS-CZE) was developed for the determination of secbumeton (SEC) in environmental water samples. The effects of diode array wavelength, buffer pH and concentration, the type and concentration of leading and terminating electrolyte, applied voltage and injection time were investigated thoroughly. The optimum background electrolyte was 100 mM formate (pH 2.5), hydrodynamic injection of 100 mM sodium chloride (30 s, 50 mbar) as leading electrolyte, electrokinetic injection of the sample (250 s, 7 kV), hydrodynamic injection of 100 mM Tris (40 s, 50 mbar) as terminating electrolyte and then a voltage of 25 kV for separation (+25 kV). Under the optimum conditions, the sensitivity of SEC was enhanced 3847-fold and 2267-fold when compared to normal hydrodynamic injection (HDI) and electrokinetic injection (EKI), respectively. Wide linear range (0.1–500 μg L-1) with good linearity, R2=0.9997, satisfactory repeatability (% RSD < 4%, n=5) and good recovery (85.7–105.6%) were obtained for SEC. The detection limit for SEC with this EKS-CZE system was 0.03 μg L-1. The results demonstrated that the developed method has great potential for the determination of SEC in complicated aqueous matrices.
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Keywords
Electrokinetic supercharging in capillary electrophoresis , for the on-line preconcentration of secbumeton in water samples