Electrokinetic Supercharging In Capillary Electrophoresis For The On-Line Preconcentration Of Secbumeton In Water Samples

dc.contributor.authorSoeung, Rasmey
dc.date.accessioned2017-10-09T01:43:37Z
dc.date.available2017-10-09T01:43:37Z
dc.date.issued2017
dc.description.abstractIn this study, an on-line preconcentration method termed electrokinetic supercharging capillary zone electrophoresis (EKS-CZE) was developed for the determination of secbumeton (SEC) in environmental water samples. The effects of diode array wavelength, buffer pH and concentration, the type and concentration of leading and terminating electrolyte, applied voltage and injection time were investigated thoroughly. The optimum background electrolyte was 100 mM formate (pH 2.5), hydrodynamic injection of 100 mM sodium chloride (30 s, 50 mbar) as leading electrolyte, electrokinetic injection of the sample (250 s, 7 kV), hydrodynamic injection of 100 mM Tris (40 s, 50 mbar) as terminating electrolyte and then a voltage of 25 kV for separation (+25 kV). Under the optimum conditions, the sensitivity of SEC was enhanced 3847-fold and 2267-fold when compared to normal hydrodynamic injection (HDI) and electrokinetic injection (EKI), respectively. Wide linear range (0.1–500 μg L-1) with good linearity, R2=0.9997, satisfactory repeatability (% RSD < 4%, n=5) and good recovery (85.7–105.6%) were obtained for SEC. The detection limit for SEC with this EKS-CZE system was 0.03 μg L-1. The results demonstrated that the developed method has great potential for the determination of SEC in complicated aqueous matrices.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/4840
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectElectrokinetic supercharging in capillary electrophoresisen_US
dc.subjectfor the on-line preconcentration of secbumeton in water samplesen_US
dc.titleElectrokinetic Supercharging In Capillary Electrophoresis For The On-Line Preconcentration Of Secbumeton In Water Samplesen_US
dc.typeThesisen_US
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