Study On Apolipoprotein E (Apoe) Gene Polymorphism And Coronary Artery Disease Biomarker In Diabetic Patients

Simple item page
dc.contributor.authorAbdul Sani, Athirah
dc.date.accessioned2017-10-05T00:36:21Z
dc.date.available2017-10-05T00:36:21Z
dc.date.issued2016
dc.description.abstractApolipoproteion E (APOE) gene plays a major role in lipoprotein metabolism and lipid transport. Various studies on the association of the APOE gene polymorphism among diabetes patients with coronary artery disease (CAD) also has been investigated in the last few years. However, there is no report among the Malaysian population in regard to the association of APOE gene polymorphism among patients with T2DM and CAD patients. Thus, the objective was to study role of APOE gene polymorphisms in development of CAD among type 2 diabetes mellitus (T2DM) patients in Hospital Kuala Lumpur (HKL). Total of 45 T2DM patients (21 men and 23 women) was investigated in which 11 of them had complications with CAD. Biochemical analyses included glucose, HbA1c and lipid profile which consists of total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL-C) and high density lipoprotein (HDL-C). Data were obtained from the Hospital Kuala Lumpur Pathology Laboratory database. Genotyping of APOE was done by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The PCR product was digested with restriction enzymes. Fragments were separated using agarose gel electrophoresis. The T2DM with and without CAD subjects had significant differences in glucose and TG plasma levels (p = 0.048 and p = 0.019). The APOE genotypes detected in this study were of ε3/ε3, ε3/ε4, ε2/ε3, ε2/ε4 and ε4/ε4 except for ε2/ε2 genotype. Research finding showed, frequency of ε3/ε4 genotype was higher among T2DM with CAD compared to T2DM no CAD. However, there was no significant difference in distribution of the APOE genotype and alleles among both groups. Study showed there was a significant difference of HDL-C level between APOE genotype (p = 0.004) and alleles (p = 0.001). It was noted only T2DM patients with no CAD were affected with allele’s variation and HDL-C levels. Further analysis on the association of the APOE gene polymorphism and lipid profile showed there were no significant difference for TC, TG and LDL-C with genotypes and alleles. In conclusion ε4 higher in CAD and reduces HDL-C.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/4796
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectPolymorphism and coronary artery Diseaseen_US
dc.subjectbiomarker in diabetic patients.en_US
dc.titleStudy On Apolipoprotein E (Apoe) Gene Polymorphism And Coronary Artery Disease Biomarker In Diabetic Patientsen_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Institut Perubatan & Pergigian Termaju - Tesis