Demonstration of antigenic and specific whole cell proteins for methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
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Date
2002
Authors
Ismail, Noraini
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Staphylococcus aureus is the most common and important cause of life threatening
bacterial infection with a high mortality rate. The absence of a positive culture represents
a dilemma in diagnosis and treatment, therefore an alternative method that is cheap, user
friendly and rapid with high sensitivity and specificity is needed to help in the diagnosis.
This study was done to identify the presence of antigenic and specific proteins of whole
cell for Staphylococcus aureus, which can be used to develop a rapid serological test. By
applying the technique of sodium dodecyl sulphate-polycarylamide gel electrophoresis
(SDS-PAGE) and Western blotting, three proteins with molecular weight of 18 kDa, 21.
5 kDa and 28 kDa were found to be antigenic and specific for methicillin-sensitive
Staphylococcus aureus. These proteins were only specific to IgM antibodies but not to
IgA and IgG antibodies. Two proteins were found to be antigenic and specific for
methicillin-resistant Staphylococcus aureus. The proteins were 57 kDa protein which was
specific to IgM antibody only and 40 kDa protein which was only specific to IgG antibody.
Description
Keywords
Staphylococcus aureus