Demonstration of antigenic and specific whole cell proteins for methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

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dc.contributor.authorIsmail, Noraini
dc.date.accessioned2017-08-25T02:16:42Z
dc.date.available2017-08-25T02:16:42Z
dc.date.issued2002
dc.description.abstractStaphylococcus aureus is the most common and important cause of life threatening bacterial infection with a high mortality rate. The absence of a positive culture represents a dilemma in diagnosis and treatment, therefore an alternative method that is cheap, user friendly and rapid with high sensitivity and specificity is needed to help in the diagnosis. This study was done to identify the presence of antigenic and specific proteins of whole cell for Staphylococcus aureus, which can be used to develop a rapid serological test. By applying the technique of sodium dodecyl sulphate-polycarylamide gel electrophoresis (SDS-PAGE) and Western blotting, three proteins with molecular weight of 18 kDa, 21. 5 kDa and 28 kDa were found to be antigenic and specific for methicillin-sensitive Staphylococcus aureus. These proteins were only specific to IgM antibodies but not to IgA and IgG antibodies. Two proteins were found to be antigenic and specific for methicillin-resistant Staphylococcus aureus. The proteins were 57 kDa protein which was specific to IgM antibody only and 40 kDa protein which was only specific to IgG antibody.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/4426
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectStaphylococcus aureusen_US
dc.titleDemonstration of antigenic and specific whole cell proteins for methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureusen_US
dc.typeThesisen_US
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