Phenolic Acid Rich Fraction Of Gynura Procumbens As Potential Antihyperlipidemic And Antioxidant Agents
Loading...
Date
2018-06
Authors
Murugesu, Kisantini
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
G. procumbens is found in most of the Southeast Asian countries and the leaves
are used as a folk medicine to treat various illnesses including hyperlipidemia. The
objectives of the present study include isolation of marker compound(s), standardization
and phytochemical analysis of G. procumbens plant samples and investigation of their
antioxidant and antihyperlipidemic effects in acute and chronic rat models,
determination of their mechanisms of action and toxicological profiles. The leaves of G.
procumbens were macerated with 95 % ethanol and fractionated into three fractions: F1
(water), F2 (50 % MeOH) and F3 (acetone). Further purification of F2 yielded a
phenolic acid, chlorogenic acid (CA). Antioxidant analyses revealed that, F2 possessed
highest phenolics content and strong antioxidant activities. Likewise, CA exhibited
potent antioxidant activities that were comparable to reference standards. The G.
procumbens plant samples, extract and fractions were standardized using phenolic acids
(caffeoylquinic acids: CA, 3,4DC, 3,5DC and 4,5DC) as marker compounds by a
validated RP-HPLC method. Phytochemical analysis revealed that, F2 was enriched
with caffeoylquinic acids compared to other fractions. Acute antihyperlipidemic study of
standardized extract, fractions and CA of G. procumbens indicated that S(F2) and CA
had most potent antihyperlipidemic activity on lowering TC and TG levels in p-407 (500
mg/kg; i.p.) induced acute hyperlipidemic rats. In addition, S(F2) also significantly
reduced levels of LDL-C, VLDL-C, AI and CRI after 58 h treatment followed by CA.
Hence S(F2) was chosen for further investigations in chronic hyperlipidemic model.
Five weeks of treatment with various doses of S(F2) resulted in dose dependent
antihyperlipidemic effects, whereby the most potent effect was exerted by 500 mg/kg of
S(F2).
Description
Keywords
Phenolic acid rich fraction of gynura procumbens as , potential antihyperlipidemic and antioxidant agents