Phenolic Acid Rich Fraction Of Gynura Procumbens As Potential Antihyperlipidemic And Antioxidant Agents

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dc.contributor.authorMurugesu, Kisantini
dc.date.accessioned2019-03-05T00:39:09Z
dc.date.available2019-03-05T00:39:09Z
dc.date.issued2018-06
dc.description.abstractG. procumbens is found in most of the Southeast Asian countries and the leaves are used as a folk medicine to treat various illnesses including hyperlipidemia. The objectives of the present study include isolation of marker compound(s), standardization and phytochemical analysis of G. procumbens plant samples and investigation of their antioxidant and antihyperlipidemic effects in acute and chronic rat models, determination of their mechanisms of action and toxicological profiles. The leaves of G. procumbens were macerated with 95 % ethanol and fractionated into three fractions: F1 (water), F2 (50 % MeOH) and F3 (acetone). Further purification of F2 yielded a phenolic acid, chlorogenic acid (CA). Antioxidant analyses revealed that, F2 possessed highest phenolics content and strong antioxidant activities. Likewise, CA exhibited potent antioxidant activities that were comparable to reference standards. The G. procumbens plant samples, extract and fractions were standardized using phenolic acids (caffeoylquinic acids: CA, 3,4DC, 3,5DC and 4,5DC) as marker compounds by a validated RP-HPLC method. Phytochemical analysis revealed that, F2 was enriched with caffeoylquinic acids compared to other fractions. Acute antihyperlipidemic study of standardized extract, fractions and CA of G. procumbens indicated that S(F2) and CA had most potent antihyperlipidemic activity on lowering TC and TG levels in p-407 (500 mg/kg; i.p.) induced acute hyperlipidemic rats. In addition, S(F2) also significantly reduced levels of LDL-C, VLDL-C, AI and CRI after 58 h treatment followed by CA. Hence S(F2) was chosen for further investigations in chronic hyperlipidemic model. Five weeks of treatment with various doses of S(F2) resulted in dose dependent antihyperlipidemic effects, whereby the most potent effect was exerted by 500 mg/kg of S(F2).en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7815
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectPhenolic acid rich fraction of gynura procumbens asen_US
dc.subjectpotential antihyperlipidemic and antioxidant agentsen_US
dc.titlePhenolic Acid Rich Fraction Of Gynura Procumbens As Potential Antihyperlipidemic And Antioxidant Agentsen_US
dc.typeThesisen_US
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