Pusat Pengajian Sains Kajihayat - Tesis

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The effects of sublethal doses of carbaryl on the biology of Dysdercus cingulatus (Fabr.)(Hemiptera; Heteroptera; Pyrrhocoridae
(1976)
Chung Gait Fee
The effects of sublethal doses of carbaryl on the biology of Oysdercus cingulatus (Fabr.) were investigated in the laboratory. The comparative toxicity of carbaryl against various life-stages of Q. cingulatus (Fabr.) as shown by L050 value was found to be first instar < second instar < third instar < fourth instar < adult male < fifth instar< adult female < gravid female, in increasing order.
Some Biochemical Aspects Of Tyrosine Aminotransferase (L-Tyrosine: 2-Oxoglutarate Aminotransferase, E.C. 2.6.1.5) (TAT) In The Stages Of Development Of Aedes Aegypti Linnaeus
(1976-04)
Chan Guat Lian
The variation of tyrosine-2-oxoglutarate amino-transferase (EC.2.6.1.5), TAT and soluble protein was charted throughout the postembryonic development and young adult growth of the mosquito, Aedes aegypti (Linnaeus).•. A close correspondence between the enzyme activity and soluble protein content was observed during the immature stages of development. Peak transaminase activity was encountered in· the middle fourth larval instar followed by a decline in activity prior to pupation. At its maximum activity, the insect enzyme compared well with the adult rat liver enzyme in activity. The larval enzyme appeared to exhibit a diurnal rhythm of activity characterized by a steady increase in activity from 0600 hours to reach maximum activity at 24 hours.
Studies On The Culture Of The Estuary Grouper, Epinephelus Salmoides Maxwell (Pisces, Serranidae), In Floating Net-Cages
(1979-10)
Teng, Seng Keh
In the present investigation, certain aspects of the food and feeding, Ilutritional requirements and stocking densities of the estuary grouper (EpinepheZus saZmoides Maxwell) were studied In floating net-cages. The results obtained were further assimilated to develop culture techniques for optimizing commercial production of the fish.
Perkembangan Kaedah Kromatografi Bagi Penentuan Klorproguanil Dan Klorsikloguanil Di Dalam Plasma
(1990-12)
Mahmud, Latifah
Kaedah-kaedah kromatografi cecair dan gas yang baru telah dimajukan bagi penentuan klorproguanil dan metabolitnya, klorsikloguanil dalam plasma. Drug-drug berkenaan berserta proguanil sebagai piawai dalaman telah diekstrak daripada plasma melalui kaedah pengekstrakan fasa pepejal pada penjerap ODS (CIS) dengan dielusikan oleh metanol yang mengandungi 0.01 % dietilamina-asid asetik. Purata peratus pengembali bagi klorproguanil melalui pengekstrakan ini adalah (85 + 3.6) % dengan pekali ubahan 4.2 % manakala bagi klorsikloguanil pula adalah (60.2 ± 3.9) % dengan pekali ubahan 6.6%.
Mikotoksin Fusarium Nygamai Burgess & Trimboli, Penyebab Penyakit Besar Rabu Pada Padi
(1993-12)
Sazlina Binti Abdul Kader
Fusarium nygamai (kultur No. P27SRP) yang digunakan dalam eksperimen ini, telah dipencil daripada batang padi yang menunjukkan gejala penyakit besar rabu di Bumbung Lima, Pulau Pinang. Kultur monokonidium F. nygamai diinokulasi pada beras steril dan dieram selama 1-4 minggu. Ekstrak mikotoksin kasar (EMK) yang dihasilkan oleh patogen setiap minggu dalam kultur beras tersebut dibioasai pada larva udang (Artemia salina).
Immunological Reactivity Of Jird (Meriones Unguiculatus) To Infection
(1993-12)
Panasoponkul, Chotechuang
Filariasis is a chronic parasitic infection which is considerable public health and socioeconomic importance in many tropical and subtropical countries and remains a major health problem in many areas of the world. The present study was undertaken to elucidate the various immunoregulatory mechanisms elicited by different doses of subcutaneous infection with B. pahangi in jirds (Meriones unguiculatus)
Studies On The Mosquito Fauna Of Kerian District With Emphasis On The Mangrove Swamp Ecotype
(1994-01)
Ahmad, Abu Hassan
A survey on the mosqui to fauna of Kerian district and its adjacent area of Bandar Baharu district was taken from August 1986 to December 1990. A total of 157 species of mosquitoes belonging to 17 genera were collected using several methods. Some of the mosquitoes were found to breed only in certain specific ecological zones while others were distributed over a wider area. Aedes amesii, and Uranotaenia latera7is were confined to Cx. sitiens the mangrove swamps. The holes in the trees of the mangrove swamps were the breeding habitat for Ae. amesii. Cx. sitiens breeds in the brackish water pools and water canals that run along the bunds that separate the mangrove swamps from the land. Ur. latera7is larvae were found breeding in the crab holes in the mangrove swamps. Adults rest in these crab holes during the day. The nipah palm axils were used by several species of Aedes and Culex as their breeding habitats.
Nilai Nutrisi Kek Isirong Kelapa Sawit (PKC) Dalam Makanan Pelet Untuk Ikan Tilapla
(2002-06)
Lim, Hong Aik
Tiga eksperimen berasingan telah dijalankan untuk mengkaji beberapa aspek nilai nutrisi kek isirong kelapa sawit (PKC) dalam makanan pelet untuk ikan tilapia. Dalam Eksperimen I, keterhadaman nutrien dan ketersediaan asid amino PKC kepada ikan tilapia telah ditentukan berbanding dengan beberapa jenis bahan mentah. Diet ruj ukan dengan 40% protein kasar dan lima diet ujian yang mengandungi diet rujukan dan bahan mentah ujian dalam nisbah 70:30 telah dirumuskan. PKC telah menunjukkan keterhadaman protein ketara yang lebih tinggi berbanding dengan serbuk kacang kelisa (WBM), dan keterhadaman jirim kering, protein dan tenaga yang lebih rendah berbanding dengan bahan-bahan mentah lain.
Cytotoxicity And Cell Deatl-I Mechanisms Of Epipremnum Pinna Tum (L.) Engl. Extracts In T-47d, A Breast Carcinoma Cell Line
(2003-05)
Tan, Mei Lan
In this study, Epipremnum pinnatum (L.) Engl. extracts were shown to exhibit remarkable inhibition against the growth of T-47D, a breast carcinoma cell line. The hexane extract was the most potent (EC5o at 2.870 ug/ml) as compared to the chloroform and methanol extracts (EC5o at 5.590 ug/ml and 6.470 ug/ml, respectively). However, cell death mechanisms elicited by these three extracts were somewhat nonsimilar.
Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
(2012-07)
Murugaiah, Hemalatha
Glucoamylase enzyme is widely utilized for conversion of starch into glucose during saccharification process. Aspergillus niger van Tieghem is commonly used for glucoamylase production in submerged cultivation system (SmF) and solid state fermentation (SSF). This research was carried out to enhance glucoamylase production by mutating A. niger van Tieghem using chemical mutagen, ethyl methane sulphonate (EMS) via random mutation. Mutant ANC9 was identified as a potential mutant which produced, 1.9 and 1.6 fold higher glucoamylase activity towards cooked starch and raw starch respectively in SmF using cooked starch as substrate. Glucoamylase activity towards raw starch and cooked starch in SSF by mutant ANC9 were 44.81± 1.2 Ulg and 101.50 ±2.2 Ulg respectively compared to wild strain which was only able to produce 34.4±O.5 Ulg towards cooked starch and 79.1±2.2 Ulg towards raw starch.
Ekologi Pembiakan Dan Analisis Filogenetik Molekul Enam Spesies Keladi Hutan Reofitik (Araceae: Schismatoglottideae)
(2012-07)
Ooi, Im Hin
Enam keladi hutan reofitik dalam Schismatoglottideae, dua dari Semenanjung Malaysia (Pip to spat ha ridleyi, P perakensis) dan empat dari Borneo (P. elongata, Ooia grabowskii, Bakoa lucens, Aridarum nicolsonii) telah dipilih sebagai wakil-wakil kepada kumpulan-kumpulan tertentu. Pemerhatian lapangan tentang mekanik seludang, pendebungan, penghasilan buah, penyebaran biji benih dan penubuhan anak benih takson-takson ini telah dijalan dan dicatatkan. Jujukan dua rantau DNA kloroplas (/rwL-trnF, w«/K-3’r/7?K) telah dikaji untuk setiap spesies dengan menggunakan teknik penjujukan DNA. Filogeni takson-takson yang dibina-semula menunjukkan genus Piptospatha ialah satu kumpulan yang polifiletik. Melalui hubung filogenetik ini pertalian evolusi takson-takson sudah difahami. Pada masa yang sama, kajian ini telah menyumbang kepada persempadanan genus Piptosaptha, menghuraikan genus-genus Bakoa dan Ooia secara tidak langsung.
Optimized Production Of Poly(3-Hydroxybutyrate) And Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) By Cupriavidus Sp. Usmaa2-4 In A One-Stage Fermentation
(2013)
Nurhezreen Binti Md Iqbal
Polyhydroxyalkanoate (PHA) is a biodegradable and biocompatible thermoplastic synthesized by microorganisms as intracellular energy and carbon reserved materials. P(3HB) homopolymer is stiff and brittle while P(3HB-co-4HB) copolymer have properties ranging from crystalline to rubber-like materials which correlates with the changes in the 4HB monomer compositions. In addition, the degradation products of P(3HB-co-4HB) is non toxic and biocompatible to human body, as the degradation product of both monomers is a natural metabolite present in mammalian body.
Floristic Composition And Economic Valuation Of 2-Ha Plots Of Hill Forest In Langkawi
(2013-03)
Thesis
Floristic composition data are a valuable tool in sustainable forest and environmental management. A 2.0-ha permanent plot (200 m x 100 m) located between 300 and 750 m a.s.l. was each established at Gunung Matchingchang Forest Reserve (GMFR) and Gunung Raya Forest Reserve (GRFR) in Langkawi for the purpose of inventoring hill floral diversity. The research sites comprised of two 2.0-ha plots each of which was located in GMFR and GRFR and was further divided into fifty 20 m x 20 m subplots. In GM_FR and GRFR, there were a total of 12,530 trees and 7,967 trees respectively. All trees with abundant species, family, diameter breast height (DBH), total biomass (TB) and stumpage value were identified, assessed and recorded. The largest species in GMFR was recorded by Hydnocarpus filipes (Flacoutiaceae) with as many as 2,591 individuals. In GRFR, Diospyros ismailii (Ebenaceae) recorded the highest number with 799 individuals.
Effect Of Varying Dietary Protein Levels In Snakehead Broodstock, Channa Striata (Bloch, 1793) On Egg, Larval Quality, Digestive Enzyme Activity And Ontogenic Development Of The Digestive Tract
(2016-01)
Manogoran, Janaranjani
The present study was carried out to determine the quality of eggs and larvae from channa striata broodstock that were fed different levels of dietary protein. C. Striata juveniles (mean weight 54.5 ± 2.6 g) were randomly selected and stocked in 4m x lm x im (length x width x depth) canvas tanks at adensity of 60 fishes per tank in triplicates for each treatment. Four experimental diets containing varying protein levels (350, 400, 450 and 500 g kg'1) were formulated and fed twice daily to satiation, at 10:00 a.M. And 17:00 p.M. For 6 months until spawning. After six months on the experimental diets, matured male and female brood fish were randomly selected for artificial spawning at the ratio of 1: 1. A series of two experiments were conducted to achieve the objectives of this cunent study. First, the quality of eggs and larvae were assessed based on biometric of egg and larvae, egg lysozyme, tolerance of larvae to starvation, tolerance to stress triggers such as temperature and ph and amino acid content.
Growth And Biochemical Composition Of Isochrysis Maritima Billard And Gayral 1972 Under Photoautotrophic And Heterotrophic Conditions : Assessment For Aquaculture Feed
(2016-08)
Eshak, Mohammad Basri
The effects of photoautotrophic and heterotrophic conditions on growth and biochemical composition of isochrysis maritima, isolated from teluk aling, penang were investigated. Under photoautotrophic condition, i. Maritima was cultivated in different nitrogen sources (nitrate, nitrite, and urea) and harvested at different growth phases (exponential, early stationary and late stationary) to examine the impact of nitrogen sources and growth phases on growth and biochemical composition. The results revealed that urea at late stationary was the most suitable nitrogen source, which recorded high cell growth (10.9 ± 0.63 x 106 cell.Ml'1) and biochemical composition including protein (32.1 ± 3.17%), carbohydrate (37.0 ± 0.36%) and fatty acids content (total sfa = 32.92 ± 2.59 %; total mufa = 6.26 ± 0.83%; total pufa = 15.55 ± 1.49%) and was further investigated onto higher volume of walne’s medium (50 l). Under heterotrophic condition, i. Maritima was cultivated in different organic carbon sources (glucose, sucrose, lactose, fructose and sodium acetate) at different concentrations (0.02 m, 0.05 m and 1.0 m) with urea as nitrogen source to determine the suitable carbon sources and concentrations to maximize the growth of this strain.
Length-Weight, Condition Factor And Feeding Guild Periophthalmus Chrysospilos (Bleeker, 1852) (Gobiidae: Oxudercinae), From Bayan Bay Coastal Water, Penang
(2016-08)
Abdullah, Mohd Ilman Che
A study on population dynamics and feeding biology of mudskipper, periophthalmus chrysospilos was conducted in bayan bay coastal water, penang from july 2013 to july 2014. This study comprised of three components: (i) length-weight relationships (lwr) and fulton condition factor (k); (ii) growth, mortality, and recruitment pattern, and (iii) stomach content analyses. The main objectives of this study are: (i) to investigate the length-weight relationships of p. Chrysospilos in different sexes and months, (ii) to elucidate the fulton condition factor and its relation to the monthly variations and water physio-chemical parameters, (iii) to study the growth parameters, mortality and recruitment pattern of p. Chrysospilos and finally (iv) to determine the feeding activities and feeding guild of p. Chrysospilos according to different sex, months and water tide. Results show the exponential values (zj) of the lwr for all individuals (n = 714), male and female fish, and fish caught during dry and rainy months were greater than 3, indicating of positive allometric growth. The fulton’s condition factor (k) for all individuals, male, female, and fish caught in dry and rainy months were 0.998 ± 0.121, 0.9907 ± 0.106, 1.008 ±0.138, 1.018 ± 0.115 and 0.998 ± 0.123, respectively. The b and k values for male and female fish were not significantly different. The b value in dry months is significantly higher than in rainy months.
Displaying Of Green Fluorescent Protein On A Recombinant Bacteriophage Infecting Escherichia Coll Atcc 11303
(2017-03)
Ong, Jia Wei
Phage display technology allows the presentation of foreign peptides on the surface of a bacteriophage. Phage display technology is used to acquire the function of the displayed peptides which was not present on wild type bacteriophage. Using phage display technology, a recombinant phage would be constructed as a model for future manipulation. The technology was applied to display a green fluorescence protein (GFP) on tail protein of Escherichia Phage YD-2008.S to create GFP labelled recombinant bacteriophage.
Production, Purification And Characterization Of Poly(3- Hydroxybutyrate) P(3hb) Depolymerase Enzyme From Burkholderia Cepacia Dpi
(2017-04)
Azami, Nor Azura
The biodegradation of polyhydroxyalkanoates (PHAs) by microbial and enzymatic methods attracts much attention since the ranges of potential applications of PHAs grow wider. However, there are limited reports on the isolation of poly(3- hydroxybutyrate) P(3HB)-degrading bacteria and detail study on the production of P(3HB) depolymerase enzyme by microorganisms from Malaysian environments.
Relationship Of Oil Palm Inflorescence And Evaluation Of Several Insecticides On Tirathaba Mundella Walker Lepidoptera Pyralidae And Elaeidobius Kamerunicus Faust Coleoptera Curculionidae
(2017-07)
Shukor, Muhammad Idrus
Oil palm plant, (Elaies guineensis Jacq.) from family Palmae was originated from the West Africa and was brought into Malaysia by British Colony. It is now known as the “golden crop” and have become one of the most important oil producing plants in the world. Oil palm inflorescences and bunches are important diet for two insects, the fruit bunch moth, Tirathaba mundella and the pollinating weevil, Elaeidobius kamerunicus. T. mundella cause 50 % loses of fresh fruit bunch (FFB) while E. kamerunicus is an important pollinator. The objectives of this study are: (1) to find the relationship between the oil palm inflorescences and bunches stage with T. mundella and E. kamerunicus, (2) to find the efficacy and residual effect of several insecticides towards the T. mundella population and (3) to determine the effect of several insecticides towards the E. kamerunicus population. By using Biologische Bundesantalt, Bundessortenamt und Chemische Industrie or BBCH scale, the oil palm inflorescences and bunches stage were described and used for further study on both insects. The results show that the young bunch (E) with new frass (N) and high infestation (H) have the highest count of larvae. Location with high rotten bunches are having high T. mundella infestation and larvae count. Besides, location with high rotten bunches also had low male inflorescence. Both results show that lower pollination which caused T. mundella infestation to increase thus increasing the rotten bunches count. The time taken for male inflorescence to complete their development was approximately 9 days. The study found that pollinating weevil populations were most abundant during peak anthesis time.
Preparation And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyhexanoate) Reinforced Kenaf Fibre
(2017-08)
Lee, Joyyi
Polyhydroxyalkanoate (PHA) is an advantageous polymeric material due to environmental friendliness but its mechanical properties limits its potential in material selection. Green composites, produced from renewable polymers such as thermoplastic PHA and natural fibres, are possible ways to solve this shortcoming. It can potentially substitute non-renewable synthetic materials in composite manufacturing sector. The polymer component of the composite, poly(3- hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] with 3HHx monomer fraction of approximately 20 mol%, was biosynthesized by fermentation using used cooking oil as the sole carbon source. Subsequently, bulk quantity ofP(3HB-co-3HHx) was recovered using solvent-free novel biological recovery method with yellow mealworms (Tenebrio molitor) and followed by further purification with water containing strong anionic detergent. P(3HB-co-3HHx) showed slightly better thermal stability than its solvent extracted derivative with the removal of inorganic compounds during the purification process.

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