The taxonomy of the freshwater featherback fish genus Chitala (Osteoglossomorpha: Notopteridae) remains unsettled because delimiting and naming Chitala species based solely on morphology led to different hypotheses. In this study, the number and identity of Chitala species present in Peninsular Malaysia were determined by employing an integrative taxonomic approach that combines morphological characters and two mitochondrial markers: the cytochrome c oxidase subunit I (COI) and cytochrome b genes.